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Diagnostic Tests – Skin Scraping and Mineral Oil Mount

1. What is this test?

A skin scraping with mineral oil mount is a bedside diagnostic test used to identify scabies. Mineral oil is applied to the suspected lesion to enhance visualization of the burrow. The skin is then scraped and examined under a microscope. The mineral oil preserves skin cells, mite feces (scybala), and allows mites to remain intact and motile, improving diagnostic yield.


2. When is this the answer?

This test is indicated when a patient presents with:


  • Intensely pruritic rash, often worse at night
  • Small linear burrows, especially in the web spaces of the hands and feet
  • Possible involvement of genitalia or breasts
  • A history suggesting close personal or sexual contact

In exam questions, the combination of pruritus + burrows + web-space involvement should immediately point to scabies, making skin scraping with mineral oil the correct diagnostic choice.

3. What is an adjuvant test often used in the same scenario?

A commonly used adjunct test is a KOH wet mount prepared from the same skin scrapings. The slide is gently heated to dissolve keratin and break up squamous cells, helping reveal mites that may be hidden beneath debris.






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